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Biochem Biophys Res Commun. 2008 Jul 18;372(1):126-30. doi: 10.1016/j.bbrc.2008.05.020. Epub 2008 May 13.

Nuclear localization of Rad52 is pre-requisite for its sumoylation.

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1
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan.

Abstract

In Saccharomyces cerevisiae, Rad52 plays major roles in several types of homologous recombination. Here, we found that rad52-K200R mutation greatly reduced sumoylation of Rad52. The rad52-K200R mutant exhibited defects in various types of recombination, such as intrachromosomal recombination and mating-type switching. The K200 residue of Rad52 is part of the nuclear localization signal (NLS), which is important for transport into the nucleus. Indeed, the addition of a SV40 NLS to Rad52-K200R suppressed the sumoylation defect of Rad52-K200R. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation.

PMID:
18482582
DOI:
10.1016/j.bbrc.2008.05.020
[Indexed for MEDLINE]
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