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Biochem Biophys Res Commun. 2008 Jul 18;372(1):108-13. doi: 10.1016/j.bbrc.2008.04.188. Epub 2008 May 13.

Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells.

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Korea Food Research Institute, Functional Food Research Center, San 46-1, Baekhyun-dong, Bundang-ku, Songnam, Kyungki-do 463-746, Republic of Korea.


Inhibition of adipocyte differentiation is one approach among the anti-obesity strategies. This study demonstrates that vitisin A, a resveratrol tetramer, inhibits adipocyte differentiation most effectively of 18 stilbenes tested. Fat accumulation and PPARgamma expression were decreased by vitisin A in a dose-dependent manner. Vitisin A significantly inhibited preadipocyte proliferation and consequent differentiation within the first 2 days of treatment, indicating that the anti-adipogenic effect of vitisin A was derived from anti-proliferation. Based on cell cycle analysis, vitisin A blocked the cell cycle at the G1-S phase transition, causing cells to remain in the preadipocyte state. Vitisin A increased p21 expression, while the Rb phosphorylation level was reduced. Therefore, vitisin A seems to induce G1 arrest through p21- and consequent Rb-dependent suppression of transcription. On the other hand, ERK and Akt signaling pathways were not involved in the anti-mitotic regulation by vitisin A. Taken together, these results suggest that vitisin A inhibits adipocyte differentiation through preadipocyte cell cycle arrest.

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