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Biotechniques. 2008 Apr;44(4):555-6, 558. doi: 10.2144/000112672.

A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples.

Author information

1
Department of Forensic Medicine, Yonsei University College of Medicine, Yonsei University, Seoul, Korea.

Abstract

To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.

PMID:
18476821
DOI:
10.2144/000112672
[Indexed for MEDLINE]
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