Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2008 Jun;5(6):535-8. doi: 10.1038/nmeth.1210. Epub 2008 May 11.

Next-generation high-density self-assembling functional protein arrays.

Author information

1
Harvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.

Abstract

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

PMID:
18469824
PMCID:
PMC3070491
DOI:
10.1038/nmeth.1210
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center