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Nat Methods. 2008 Jun;5(6):527-9. doi: 10.1038/nmeth.1211. Epub 2008 May 11.

Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.

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1
Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.

Abstract

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

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PMID:
18469823
DOI:
10.1038/nmeth.1211
[Indexed for MEDLINE]
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