Format

Send to

Choose Destination
EMBO J. 1991 Feb;10(2):449-57.

Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.

Author information

1
Cold Spring Harbor Laboratory, Cold Spring Harbor 11724.

Abstract

Bovine papillomavirus (BPV) DNA is maintained as an episome with a constant copy number in transformed cells and is stably inherited. To study BPV replication we have developed a transient replication assay based on a highly efficient electroporation procedure. Using this assay we have determined that in the context of the viral genome two of the viral open reading frames, E1 and E2, are required for replication. Furthermore we show that when produced from expression vectors in the absence of other viral gene products, the full length E2 transactivator polypeptide and a 72 kd polypeptide encoded by the E1 open reading frame in its entirety, are both necessary and sufficient for replication BPV in C127 cells.

PMID:
1846806
PMCID:
PMC452666
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center