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J Food Prot. 2008 Apr;71(4):839-44.

Use of single-strand conformation polymorphism of amplified 16S rDNA for grouping of bacteria isolated from foods.

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Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato, Tokyo 108-8477, Japan.


The grouping method for isolated strains from foods using single-strand conformation polymorphism (SSCP) after PCR amplification of a portion of 16S rDNA was developed. This method was able to group the strains from various food samples based on 16S rDNA sequence. As 97.8% of the isolated strains from various foods were grouped correctly, use of the PCR-SSCP method enables the prompt and labor-saving analysis of microbial population of food-derived bacterial strains. Advantages in speed and accuracy of bacterial population identification by the PCR-SSCP method have practical application for food suppliers and testing laboratories.

[Indexed for MEDLINE]

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