The objective of this study was to investigate the effects of whole-body vibration on multiple tissues simultaneously in fourteen healthy women. On three separate days, participants were exposed to frequencies, 3, 4.5, or 6 Hz (at 0.9 g(r.m.s) acceleration in vertical direction) per day on a simulator for 16 min. While sitting 'with' and 'without' backrest support, participants also performed handgrip contractions for 1 min. Cerebral and lumbar muscle oxygenation and blood volume responses were measured using near-infrared spectroscopy. Cardiorespiratory responses were collected using a metabolic cart. In general, cerebral and cardiorespiratory responses increased with vibration compared to without vibration, whereas in the lumbar region oxygenation and blood volume responses decreased. Greatest cerebral responses were observed at 6 Hz (P<0.05). When compared to exposure to vibration without performing work, significant decrease in lumbar responses was observed during handgrip contractions in both conditions of sitting 'with' and 'without' a backrest (P<0.05). Such decreases in the lumbar responses suggest postural load due to prolonged sitting combined with physical activity during vibration, might reduce vascular supply to the paraspinal muscles. This study reiterates the importance of understanding the physiological basis for various health disorders in women due to exposure to whole-body vibration.