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Anal Quant Cytol Histol. 2008 Feb;30(1):47-52.

MIB-1 counting methods in meningiomas and agreement among pathologists.

Author information

1
Department of Pathology, Izmir Ataturk Education and Research Hospital, Izmir, Turkey. rezankoa@yahoo.com

Abstract

OBJECTIVE:

To evaluate the correlation of MIB-1 labeling index (LI) obtained by 2 counting methods with histologic grade and investigate interobserver variability between these methods.

STUDY DESIGN:

A total of 65 meningiomas were analyzed for proliferation with 2 counting methods by 2 pathologists using MIB-1 antibody. In the first method, the most densely staining areas were counted (HL method). In the second method, randomly selected areas were counted (RS method).

RESULTS:

MIB-1 values correlated well with histologic grade in both methods. As expected, the tumors with recurrence had significantly higher LIs than the nonrecurrent tumors in each method. However, there was a statistically significant difference in the mean MIB-1 values of between the HL and RS methods. When MIB-1 LI was compared between 2 pathologists, perfect agreement in the HL method and substantial agreement in the RS method were achieved.

CONCLUSION:

Our results showed that values of MIB LIs differ with different counting methods. Nonetheless, both methods showed good correlation with World Health Organization grades. Therefore standardization of 1 counting method is of great importance for determining a reliable and specific cutoff value in assessing the risk of recurrence in meningiomas.

PMID:
18459587
[Indexed for MEDLINE]

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