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Opt Lett. 2008 May 1;33(9):1020-2.

Determination of the three-dimensional orientation of single molecules.

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Blackett Laboratory, Department of Physics, Imperial College London, Prince Consort Road, London SW7 2BZ, UK.


A simple method for real-time determination of the full three-dimensional orientation of the emission dipole of single molecules is presented. Introduction of a pi phase step in the back focal plane of the collector lens breaks the inherent symmetry, allowing the longitudinal dipole component to be measured. Experimental tolerances and the consequence of a nonzero bandwidth emission spectrum are also discussed. The scheme also allows for the longitudinal component of an electric field to be found experimentally.


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