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Pflugers Arch. 2008 Sep;456(6):1229-37. doi: 10.1007/s00424-008-0478-5. Epub 2008 May 1.

PKC stimulated by glucagon decreases UT-A1 urea transporter expression in rat IMCD.

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Laboratório de Pesquisa Básica-LIM 12, Hospital das Clínicas da Faculdade de Medicina-Nefrologia, Universidade de São Paulo, Av Dr Arnaldo, 455, São Paulo, SP, 01246-903, Brasil.


It is well-known that glucagon increases fractional excretion of urea in rats after a protein intravenous infusion. This effect was investigated by using: (a) in vitro microperfusion technique to measure [(14)C]-urea permeability (Pu x 10(-5)cm/s) in inner medullary collecting ducts (IMCD) from normal rats in the presence of 10(-7)M of glucagon and in the absence of vasopressin and (b) immunoblot techniques to determine urea transporter expression in tubule suspension incubated with the same glucagon concentration. Seven groups of IMCDs (n = 47) were studied. Our results revealed that: (a) glucagon decreased urea reabsorption dose-dependently; (b) the glucagon antagonist des-His(1)-[Glu(9)], blocked the glucagon action but not vasopressin action; (c) the phorbol myristate acetate, decreased urea reabsorption but (d) staurosporin, restored its effect; e) staurosporin decreased glucagon action, and finally, (f) glucagon decreased UT-A1 expression. We can conclude that glucagon reduces UT-A1 expression via a glucagon receptor by stimulating PKC.

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