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Fungal Genet Biol. 2008 Jun;45(6):922-32. doi: 10.1016/j.fgb.2008.03.009. Epub 2008 Mar 25.

Aspergillus oryzae atfB encodes a transcription factor required for stress tolerance in conidia.

Author information

1
National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima, Japan. k.sakamoto@nrib.go.jp

Abstract

Using an Aspergillus oryzae EST database, we identified a gene encoding a transcription factor (atfB), which is a member of the ATF/CREB family. Expression of atfB was barely detectable during vegetative growth, but was readily detected during conidiation in solid-state culture. Microarray analyses showed that expression of many other genes, including catalase (catA), were downregulated in an atfB-disruptant. The expression of most of these genes was upregulated in the wild-type strain during the conidiation phase in solid-state culture, and the expression pattern was similar to that of atfB itself. In the absence of stress, e.g. heat-shock or hydrogen peroxide, the conidial germination ratios for the DeltaatfB strain and the wild-type strain were similar, but the stress tolerance of conidia carrying the DeltaatfB deletion was less than that of the wild-type conidia. CRE-like DNA motifs, which are bound by ATF/CREB proteins, were found in the promoters of most of the downregulated genes in the DeltaatfB strain. Thus, atfB appears to encode a transcription factor required for stress tolerance in conidia.

PMID:
18448366
DOI:
10.1016/j.fgb.2008.03.009
[Indexed for MEDLINE]

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