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Appl Environ Microbiol. 2008 Jul;74(13):4226-30. doi: 10.1128/AEM.00013-08. Epub 2008 Apr 25.

Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.

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1
Department of Environmental Health, School of Public Health, Seoul National University, 28 Yunkeun-Dong, Chongro-Ku, Seoul 110-799, South Korea.

Abstract

We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.

PMID:
18441102
PMCID:
PMC2446532
DOI:
10.1128/AEM.00013-08
[Indexed for MEDLINE]
Free PMC Article
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