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Fertil Steril. 2009 Apr;91(4):1230-7. doi: 10.1016/j.fertnstert.2008.01.075. Epub 2008 Apr 28.

Chromosomal instability in rhesus macaque preimplantation embryos.

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  • 1Departments of Obstetrics & Gynecology and Physiology, CS Mott Center for Human Growth and Development, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA. cdupont@med.wayne.edu

Abstract

OBJECTIVE:

To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos.

DESIGN:

Prospective rhesus monkey IVF study.

SETTING:

Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center.

ANIMAL(S):

Young rhesus macaque females.

INTERVENTION(S):

In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes.

MAIN OUTCOME MEASURE(S):

Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established.

RESULT(S):

Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid.

CONCLUSION(S):

These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.

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