Format

Send to

Choose Destination
See comment in PubMed Commons below
Reprod Toxicol. 2008 Apr;25(3):374-80. doi: 10.1016/j.reprotox.2008.02.006. Epub 2008 Mar 18.

Effects of blue-green algal toxin cylindrospermopsin (CYN) on human granulosa cells in vitro.

Author information

  • 1Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, Adelaide, South Australia 5042, Australia. Fiona.Young@flinders.edu.au

Abstract

The blue-green algal toxin cylindrospermopsin (CYN) occurs in public water supplies. CYN was hepatotoxic when administered orally to mice, and cytotoxic and genotoxic to human cell lines. To determine the effects of CYN on primary human IVF-derived granulosa cells, 0-1 microg/ml CYN was added to cells for 2, 4 or 6h+/-hCG (n=6), or for 24, 48 and 72 h (n=6). Cytotoxicity was evaluated by MTT assay, and secreted progesterone or estrogen quantified by radioimmunoassay. 24h exposure to 1 microg/ml CYN was cytotoxic (p<0.05), whereas 0.0625 microg/ml CYN did not cause cytotoxicity or affect estrogen production, but did inhibit basal progesterone production (p<0.01). Similarly, 6h exposure to 1 microg/ml CYN did not affect cytotoxicity or hCG-stimulated estrogen production, but did inhibit hCG-stimulated progesterone production (p<0.01). In this in vitro assay, CYN inhibited progesterone production and therefore has the potential to be an endocrine disrupter by changing the progesterone:estrogen ratio in women.

PMID:
18440200
DOI:
10.1016/j.reprotox.2008.02.006
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center