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Biochem Biophys Res Commun. 2008 Jun 27;371(2):267-72. doi: 10.1016/j.bbrc.2008.04.068. Epub 2008 Apr 24.

Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1.

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  • 1Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, 5-1, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.

Abstract

Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.

PMID:
18439910
DOI:
10.1016/j.bbrc.2008.04.068
[PubMed - indexed for MEDLINE]
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