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Biotechnol Bioeng. 2008 Jun 15;100(3):599-603. doi: 10.1002/bit.21794.

N-Acyl homoserine lactonase promotes prevention of Erwinia virulence with zwittermicin A-producing strain Bacillus cereus.

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1
State Key Laboratory of Agricultural Microbiology, College of Life Science & Technology, Huazhong Agricultural University, Wuhan 430070, PR China.

Abstract

Zwittermicin A (ZwA), a hybrid polyketide- peptide antibiotic, and N-acyl -homoserine-lactonase (AHL-lactonase), a kind of quorum-quenching enzyme, have the potential to prevent the virulence of Erwinia carotovora, which is a major pathogen of soft rot. The purpose of this study was to combine the two agents in one host to improve their prevention efficacy. AHL-lactonase was over-expressed in the ZwA-producing strain Bacillus cereus and derivates were identified to create genetically modified (GM) strains. The comparative results showed that neither ZwA nor AHL-lactonase impacted the yield of the other; the increased yield of ZwA could promote the prevention efficacy of GM strains; Coexistence of ZwA and AHL-lactonase in the GM strains had better prevention efficacy than either has separately. It is a potential therapy to provide more effective prevention and withstand gradually increasing pesticide-resistance by combining the two antibacterial agents.

PMID:
18438870
DOI:
10.1002/bit.21794
[Indexed for MEDLINE]

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