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Mol Imaging Biol. 2008 Jul-Aug;10(4):182-91. doi: 10.1007/s11307-008-0136-5. Epub 2008 Apr 25.

Genome-free viral capsids as carriers for positron emission tomography radiolabels.

Author information

1
Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA.

Abstract

PURPOSE:

We have developed a modular synthetic strategy to append imaging agents to a viral capsid.

PROCEDURES:

The hollow protein shell of bacteriophage MS2 (mtMS2) was labeled on its inside surface with [18F]fluorobenzaldehyde through a multistep bioconjugation strategy. An aldehyde functional group was first attached to interior tyrosine residues through a diazonium coupling reaction. The aldehyde was further elaborated to an alkoxyamine functional group, which was then condensed with n.c.a. [18F]fluorobenzaldehyde. Biodistribution of the radioactive MS2 conjugates was subsequently evaluated in Sprague-Dawley rats.

RESULTS:

Relative to fluorobenzaldehyde, fluorine-18-labeled MS2 exhibited prolonged blood circulation time and a significantly altered excretion profile. It was also observed that additional small molecule cargo installed inside the capsids did not alter the biodistribution.

CONCLUSIONS:

These studies provide further insight into the pharmacokinetic behavior of nanomaterials and serve as a platform for the future development of targeted imaging and therapeutic agents based on mtMS2.

PMID:
18437498
DOI:
10.1007/s11307-008-0136-5
[Indexed for MEDLINE]

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