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Curr Protoc Immunol. 2007 Aug;Chapter 6:Unit 6.30. doi: 10.1002/0471142735.im0630s78.

Measurement of interleukin-21.

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  • 1National Institutes of Health, Bethesda, Maryland, USA.

Abstract

This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8(+) T cells using a flow cytometry-based analysis is described.

PMID:
18432995
DOI:
10.1002/0471142735.im0630s78
[PubMed - indexed for MEDLINE]

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