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Curr Protoc Immunol. 2004 Feb;Chapter 8:Unit 8.9. doi: 10.1002/0471142735.im0809s58.

Staining proteins in gels.

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  • 1Shriners Hospital for Crippled Children, Tampa, Florida, USA.


This unit describes protocols for detecting protein in a gel by either Coomassie blue, silver or fluorescent staining. Alternate rapid staining procedures are provided for each method and a support protocol describes how to photograph stained gels. Fluorescent staining (e.g., with SYPRO Orange or Red) is described as a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Staining of proteins in SDS-polyacrylamide gels is described, and an alternate protocol details variations in the procedure for proteins in nondenaturing gels. A final support protocol describes the photography of fluorescently stained proteins.

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