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Curr Protoc Immunol. 2004 May;Chapter 3:Unit 3.12. doi: 10.1002/0471142735.im0312s60.

Proliferative assays for T cell function.

Author information

1
Netherlands Cancer Institute, Amsterdam, The Netherlands.

Abstract

This addition to UNIT 3.12 will describe the assays needed to evaluate CD4.CD25.T cell non-responsiveness and function. Unlike the conventional T cells described in , Basic Protocol 1, CD4.CD25. cells do not proliferate to TCR stimuli alone. The conditions required to induce proliferation are described. This addition will also describe the assay in which CD4.CD25.T cells are co-cultured with conventional T cells in order to assess their suppressive function. Finally, this addition will describe the culture conditions for the activation and expansion of CD4.CD25. cells.

PMID:
18432927
DOI:
10.1002/0471142735.im0312s60
[Indexed for MEDLINE]

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