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Curr Protoc Immunol. 2001 May;Chapter 17:Unit 17.2. doi: 10.1002/0471142735.im1702s35.

Monitoring antigen-specific T cells using MHC-Ig dimers.

Author information

1
Johns Hopkins University, Baltimore, Maryland, USA.

Abstract

The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.

PMID:
18432743
DOI:
10.1002/0471142735.im1702s35
[Indexed for MEDLINE]

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