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Curr Protoc Protein Sci. 2003 Feb;Chapter 21:Unit 21.13. doi: 10.1002/0471140864.ps2113s30.

Expression, purification, and characterization of caspases.

Author information

1
The Burnham Institute, La Jolla, California, USA.

Abstract

This unit describes a protocol to obtain milligram amounts of enzymatically active pure recombinant caspases. Specific details for the expression, purification of caspase-3, -6, -7, -8, -9 and -10 are discussed along with strategies to obtain particular forms (e.g., the zymogen) of some of them.

PMID:
18429241
DOI:
10.1002/0471140864.ps2113s30
[Indexed for MEDLINE]

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