Format

Send to

Choose Destination
Curr Protoc Nucleic Acid Chem. 2003 May;Chapter 5:Unit 5.6. doi: 10.1002/0471142700.nc0506s12.

Synthesis of endcap dimethoxytrityl phosphoramidites for endcapped oligonucleotides.

Author information

1
Purdue University, West Lafayette, Indiana, USA.

Abstract

Endcaps may be either aromatic or aliphatic molecules that specifically cross-link the 5' end of one strand with the 3' end of the complementary strand in a DNA duplex. Endcaps may be viewed as a replacement of the loop region nucleotides of a DNA hairpin, with the added advantage of increased thermal stability. An endcap is incorporated into the sequence during oligonucleotide synthesis. Three endcaps are described in this unit. The naphthalene diimide endcap prefers to base stack with GC base pairs. The terthiophene endcap has higher lipophilicity than the naphthalene diimide endcap and provides higher stability when stacked over an AT base pair. The 2,2'-oxydiacetamide endcap provides lower enhancement in stability but a more rigid and well-defined structure than the oligo(ethylene glycol) endcaps. Synthesis of endcapped oligonucleotides can be carried out using standard automated synthesis protocols with only minor modifications.

PMID:
18428911
DOI:
10.1002/0471142700.nc0506s12
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center