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Curr Protoc Nucleic Acid Chem. 2001 May;Chapter 4:Unit 4.6. doi: 10.1002/0471142700.nc0406s03.

3'-modified oligonucleotides and their conjugates.

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  • 1Biosearch Technologies, Inc., Novato, California, USA.

Abstract

Solid-phase synthesis of 3'-aminoalkyl, 3'-thioalkyl, and 3'-polyethyleneglycol are described. The first two are important as specific points of attachment for a large variety of reporter groups; the latter is important because of its increased cell membrane permeability, which aids in the development of antisense drugs. These protocols cover details of the synthetic organic chemistry needed to make each solid support, as well as DNA synthesis, workup, characterization, and conjugation.

PMID:
18428853
DOI:
10.1002/0471142700.nc0406s03
[PubMed - indexed for MEDLINE]
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