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Nat Methods. 2008 May;5(5):397-9. doi: 10.1038/nmeth.1206. Epub 2008 Apr 20.

Monovalent, reduced-size quantum dots for imaging receptors on living cells.

Author information

1
Department of Chemistry, 77 Massachusetts Avenue, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts 02139, USA.

Abstract

We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monovalency prevented EphA3 tyrosine kinase activation.

PMID:
18425138
PMCID:
PMC2637151
DOI:
10.1038/nmeth.1206
[Indexed for MEDLINE]
Free PMC Article

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