Format

Send to

Choose Destination
Vaccine. 2008 May 19;26(21):2601-14. doi: 10.1016/j.vaccine.2008.03.035. Epub 2008 Apr 9.

Canine MDCK cell lines are refractory to infection with human and mouse prions.

Author information

1
Institute of Neuropathology, University Hospital of Zürich, Schmelzbergstrasse 12, Zürich, Switzerland. magdalini.polymenidou@usz.ch

Abstract

Influenza vaccine production in embryonated eggs is associated with many disadvantages, and production in cell culture systems is a viable alternative. Madin Darby canine kidney (MDCK) cells are permissive for a variety of orthomyxoviruses and have proven particularly suitable for vaccine mass production. However, mammalian cells harboring the Prnp gene can theoretically acquire prion infections. Here, we have attempted to infect MDCK cells and substrains thereof with prions. We found that MDCK cells did not produce any protease-resistant PrP(Sc) upon exposure to brain homogenates derived from humans suffering from Creutzfeldt-Jakob disease (CJD) or from mice infected with Rocky Mountain Laboratory (RML) scrapie prions. Further, transmission of MDCK lysates to N2aPK1 cells did not induce formation of PrP(Sc) in the latter. PrP(C) biogenesis and processing in MDCK cells were similar to those of prion-sensitive N2aPK1 cells. However, steady-state levels of PrP(C) were very low, and PrP(C) did not partition with detergent-resistant membranes upon density gradient analysis. These factors may account for their resistance to infection. Alternatively, prion resistance may be related to the specific sequence of canine Prnp, as suggested by the lack of documented prion diseases in dogs.

PMID:
18423803
DOI:
10.1016/j.vaccine.2008.03.035
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science Icon for Zurich Open Access Repository and Archive
Loading ...
Support Center