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Cytotherapy. 2008;10(3):243-53. doi: 10.1080/14653240801950000.

Immunophenotype characterization of rat mesenchymal stromal cells.

Author information

1
Department of Pediatric Surgery, University of Texas Medical School at Houston, Houston, TX 77030, USA.

Abstract

BACKGROUND:

Mesenchymal stromal cells (MSC) have shown diverse therapeutic potential. While characterization of human and mouse MSC has seen significant advances, rat bone marrow-derived MSC (rBM-MSC) remain under-characterized. We detail the isolation, expansion, differentiation, and detailed immunocharacterization of rBM-MSC.

METHODS:

Rat MSC were isolated and expanded in multipotent adult progenitor cell (MAPC) media, and cell-surface marker expression through 10 passages was used to characterize the population and multipotency was confirmed via differentiation.

RESULTS:

By passage 3, rBM-MSC were found to be CD11b-, CD45-, CD29+, CD49e+, CD73+, CD90+, CD105+ and Stro-1+, without the use of cell sorting. Media selection was responsible for the isolation of a nearly homogeneous population of rBM-MSC. The rBM-MSC immunophenotype changed by passage 10, showing decreases in CD73, CD105 and Stro-1 expression.

DISCUSSION:

Detailed characterization of cell populations facilitates accurate and reproducible cell therapy investigation. Given the expanding body of research involving rBM-MSC, these results advance our ability to compare rBM-MSC populations.

PMID:
18418770
DOI:
10.1080/14653240801950000
[Indexed for MEDLINE]

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