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Appl Biochem Biotechnol. 2008 Mar;148(1-3):245-56. doi: 10.1007/s12010-007-8119-6. Epub 2008 Jan 3.

Bacterial cellulose production by Acetobacter xylinum strains from agricultural waste products.

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Department of Biotechnology, Faculty of Applied Science, King Mongkut's Institute of Technology North Bangkok, Piboonsongkram Road, Bangsue, Bangkok, 10800, Thailand.


Bacterial cellulose is a biopolysaccharide produced from the bacteria, Acetobacter xylinum. Static batch fermentations for bacterial cellulose production were studied in coconut and pineapple juices under 30 degrees C in 5-l fermenters by using three Acetobacter strains: A. xylinum TISTR 998, A. xylinum TISTR 975, and A. xylinum TISTR 893. Experiments were carried out to compare bacterial cellulose yields along with growth kinetic analysis. Results showed that A. xylinum TISTR 998 produced a bacterial cellulose yield of 553.33 g/l, while A. xylinum TISTR 893 produced 453.33 g/l and A. xylinum TISTR 975 produced 243.33 g/l. In pineapple juice, the yields for A. xylinum TISTR 893, 975, and 998 were 576.66, 546.66, and 520 g/l, respectively. The strain TISTR 998 showed the highest productivity when using coconut juice. Morphological properties of cellulose pellicles, in terms of texture and color, were also measured, and the textures were not significantly different among treatments.

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