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Dev Cell. 2008 Apr;14(4):605-15. doi: 10.1016/j.devcel.2008.02.011.

Self-regulatory circuits in dorsoventral axis formation of the short-germ beetle Tribolium castaneum.

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1
Institute of Developmental Biology, University of Cologne, Gyrhofstrasse 17, 50931 Cologne, Germany.

Abstract

The rel/NF-kappaB transcription factor Dorsal controls dorsoventral (DV) axis formation in Drosophila. A stable nuclear gradient of Dorsal directly regulates approximately 50 target genes. In Tribolium castaneum (Tc), a beetle with an ancestral type of embryogenesis, the Dorsal nuclear gradient is not stable, but rapidly shrinks and disappears. We find that negative feedback accounts for this dynamic behavior: Tc-Dorsal and one of its target genes activate transcription of the IkB homolog Tc-cactus, terminating Dorsal function. Despite its transient role, Tc-Dorsal is strictly required to initiate DV polarity, as in Drosophila. However, unlike in Drosophila, embryos lacking Tc-Dorsal display a periodic pattern of DV cell fates along the AP axis, indicating that a self-organizing ectodermal patterning system operates independently of mesoderm or maternal DV polarity cues. Our results also elucidate how extraembryonic tissues are organized in short-germ embryos, and how patterning information is transmitted from the early embryo to the growth zone.

PMID:
18410735
DOI:
10.1016/j.devcel.2008.02.011
[Indexed for MEDLINE]
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