Objective: This study was to establish BALB/c murine model featured with the human-mouse chimeras from umbilical cord blood transplantation.
Methods: Thirty BALB/c nude mice were exposed to 350 cGy radiation under the sublethal condition. The nuclear cells from fresh umbilical cord blood were injected into the mice of experimental group via tail vein, in which the mice were further allocated to A, B and C sub-group with given 1.0 x 10(7), 2.0 x 10(7) and 3.0 x 10(7) nuclear cells per mouse respectively, and simultaneously the equal volume normal sodium (NS) was injected into the mice of control group. White blood cells from peripheral blood in experimental group and the control group were detected at week 1, 2, 3, 4 and 8 after transplantation. Human CD34+ and CD45+ from peripheral blood in experimental group were detected by using flow cytometry analysis on week 4, 6 and 8 after transplantation in order to be the human-mouse chimeras known.
Results: No difference in the number of white blood cells showed between experimental groups and control group before blood cell transplantation and after transplantation conducted on for 8 weeks (P > 0.05), but the number of white blood cells from experimental groups and control group wasn't totally same on week 1, 2, 3 and 4 after cell transplantation conducted (P < 0.05). As compared with pre-transplantation, the number of white blood cells for post-transplantation at different time in experimental groups and control group was decreasing, and the lowest on 1 week, then going up and to the level of pre-transplantation on 4 week in experimental groups and on 8 week in control group. CD34+ and CD45+ cells in peripheral blood for nude mice appeared on 4 week, but the total number was little. The number of CD34+, CD45+ cells in experimental group A was less than those in experimental group B and C at all the stage (P < 0.05), but there was no difference between experimental group B and C(P > 0.05). There was obvious correlation between CD34+ and CD45+ on 4 week (r = 0. 903, P < 0.05), but this correlation didn't appear on 6 and 8 week (P > 0.05), which might be related to the amount of sample.
Conclusion: The BALB/c murine model can be successfully established with the human-mouse chimeras from umbilical cord blood transplantation.