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J Proteome Res. 2008 Apr;7(4):1500-7. doi: 10.1021/pr7008109. Epub 2008 Apr 4.

Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.

Author information

1
Department of Pathology, Vanderbilt University, Nashville, Tennessee 37232, USA. melinda.sanders@vanderbilt.edu

Abstract

We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.

PMID:
18386930
PMCID:
PMC2738605
DOI:
10.1021/pr7008109
[Indexed for MEDLINE]
Free PMC Article

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