Format

Send to

Choose Destination
See comment in PubMed Commons below
J Virol. 2008 Jun;82(11):5573-83. doi: 10.1128/JVI.00058-08. Epub 2008 Apr 2.

Roles of LEF-4 and PTP/BVP RNA triphosphatases in processing of baculovirus late mRNAs.

Author information

  • 1Department of Entomology, Texas A&M University, 2475 TAMU, College Station, TX 77843-2475, USA.

Abstract

The baculovirus Autographa californica nucleopolyhedrovirus encodes two proteins with RNA triphosphatase activity. Late expression factor LEF-4, which is an essential gene, is a component of the RNA polymerase and also encodes the RNA capping enzyme guanylyltransferase. PTP/BVP is also an RNA triphosphatase, but is not essential for viral replication, possibly because its activity is redundant to that of LEF-4. To elucidate the role of these proteins in mRNA cap formation, a mutant virus that lacked both RNA triphosphatase activities was constructed. Infection studies revealed that the double-mutant virus was viable and normal with respect to the production of budded virus. Pulse-labeling studies and immunoblot analyses showed that late gene expression in the double mutant was equivalent to that in the wild type, while polyhedrin expression was slightly reduced. Direct analysis of the mRNA cap structure indicated no alteration of cap processing in the double mutant. Together, these results reveal that baculoviruses replicate and express their late genes at normal levels in the absence of its two different types of RNA triphosphatases.

PMID:
18385232
PMCID:
PMC2395224
DOI:
10.1128/JVI.00058-08
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center