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Prog Biophys Mol Biol. 2008 Jun-Jul;97(2-3):367-82. doi: 10.1016/j.pbiomolbio.2008.02.017. Epub 2008 Feb 16.

Cell cultures as models of cardiac mechanoelectric feedback.

Author information

1
Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA.

Abstract

Although stretch-activated currents have been extensively studied in isolated cells and intact heart in the context of mechanoelectric feedback (MEF) in the heart, quantitative data regarding other mechanical parameters such as pressure, shear, bending, etc, are still lacking at the multicellular level. Cultured cardiac cell monolayers have been used increasingly in the past decade as an in vitro model for the studies of fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level. Optical mapping makes possible multisite recording and analysis of action potentials and wavefront propagation, suitable for monitoring the electrophysiological activity of the cardiac cell monolayer under a wide variety of controlled mechanical conditions. In this paper, we review methodologies that have been developed or could be used to mechanically perturb cell monolayers, and present some new results on the acute effects of pressure, shear stress and anisotropic strain on cultured neonatal rat ventricular myocyte (NRVM) monolayers.

PMID:
18384846
PMCID:
PMC2733372
DOI:
10.1016/j.pbiomolbio.2008.02.017
[Indexed for MEDLINE]
Free PMC Article

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