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PLoS One. 2008 Apr 2;3(4):e1883. doi: 10.1371/journal.pone.0001883.

Efficient in vivo electroporation of the postnatal rodent forebrain.

Author information

1
IBDML, CNRS/Université de Méditerranée, Campus de Luminy, Marseille, France.

Abstract

Functional gene analysis in vivo represents still a major challenge in biomedical research. Here we present a new method for the efficient introduction of nucleic acids into the postnatal mouse forebrain. We show that intraventricular injection of DNA followed by electroporation induces strong expression of transgenes in radial glia, neuronal precursors and neurons of the olfactory system. We present two proof-of-principle experiments to validate our approach. First, we show that expression of a human isoform of the neural cell adhesion molecule (hNCAM-140) in radial glia cells induces their differentiation into cells showing a neural precursor phenotype. Second, we demonstrate that p21 acts as a cell cycle inhibitor for postnatal neural stem cells. This approach will represent an important tool for future studies of postnatal neurogenesis and of neural development in general.

PMID:
18382666
PMCID:
PMC2270900
DOI:
10.1371/journal.pone.0001883
[Indexed for MEDLINE]
Free PMC Article

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