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Mol Cell Biol. 2008 Jun;28(12):3894-904. doi: 10.1128/MCB.00322-08. Epub 2008 Mar 31.

CHD8 is an ATP-dependent chromatin remodeling factor that regulates beta-catenin target genes.

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Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-0606, USA.


ATP-dependent chromatin remodeling by the CHD family of proteins plays an important role in the regulation of gene transcription. Here we report that full-length CHD8 interacts directly with beta-catenin and that CHD8 is also recruited specifically to the promoter regions of several beta-catenin-responsive genes. Our results indicate that CHD8 negatively regulates beta-catenin-targeted gene expression, since short hairpin RNA against CHD8 results in the activation of several beta-catenin target genes. This regulation is also conserved through evolution; RNA interference against kismet, the apparent Drosophila ortholog of CHD8, results in a similar activation of beta-catenin target genes. We also report the first demonstration of chromatin remodeling activity for a member of the CHD6-9 family of proteins, suggesting that CHD8 functions in transcription through the ATP-dependent modulation of chromatin structure.

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