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J Am Soc Mass Spectrom. 2008 Jun;19(6):882-6. doi: 10.1016/j.jasms.2008.02.013. Epub 2008 Mar 6.

Solvent-free matrix dry-coating for MALDI imaging of phospholipids.

Author information

1
Department of Chemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.

Abstract

A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-microm stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods.

PMID:
18378160
PMCID:
PMC2696184
DOI:
10.1016/j.jasms.2008.02.013
[Indexed for MEDLINE]
Free PMC Article

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