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FEMS Microbiol Lett. 1991 Nov 1;68(1):91-5.

Confirmation by using mutant strains that the membrane-bound H(+)-ATPase is the major source of non-linear dielectricity in Saccharomyces cerevisiae.

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Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed, U.K.


Non-linear dielectric spectroscopy is a novel technique for determining the activity of (predominantly) membranous enzymes as their ability to generate harmonics when excited with a sinusoidal electrical field. In washed suspensions of yeast cells, the ability to generate harmonics is inhibited by low concentrations of sodium vanadate, suggesting that the vanadate-sensitive H(+)-ATPase is the major source of the non-linear dielectricity. This conclusion is greatly strengthened by the demonstration herein that the generation of harmonics by a strain containing a vandate-resistant H(+)-ATPase is also highly resistant to sodium metavanadate.

[Indexed for MEDLINE]

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