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Methods Mol Biol. 2008;446:267-80. doi: 10.1007/978-1-60327-084-7_19.

Immunochemical methods for the rapid screening of the o-glycosidically linked N-acetylglucosamine modification of proteins.

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1
Department of Biochemistry, Institute of Animal Sciences, University of Bonn, Bonn, Germany.

Abstract

for the rapid screening of specific post-translational modifications antibody-based methods are very well suited and applicable without demanding expenditure. Here we describe the immunochemical detection of the O-glycosidically linked cytosolic N-acetylglucosamine modification of proteins, which has attracted increasing interest in the last years. Two different monoclonal antibodies were used in enzyme-linked immunosorbent assays (ELISA), Western blots of 1- and 2- dimension (1D and 2D) separated proteins and immunohistochemical analysis of tissue sections. Slight differences in the recognition of this post-translational epitope by the 2 antibodies are observed and will be discussed.

PMID:
18373264
DOI:
10.1007/978-1-60327-084-7_19
[Indexed for MEDLINE]

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