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Cell Stem Cell. 2007 Dec 13;1(6):635-45. doi: 10.1016/j.stem.2007.10.001.

Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood.

Author information

1
Department of Internal Medicine, Division of Hematology, Stanford University, Palo Alto, CA 94304, USA. rmajeti@stanford.edu

Abstract

Mouse hematopoiesis is initiated by long-term hematopoietic stem cells (HSC) that differentiate into a series of multipotent progenitors that exhibit progressively diminished self-renewal ability. In human hematopoiesis, populations enriched for HSC activity have been identified, as have downstream lineage-committed progenitors, but multipotent progenitor activity has not been uniquely isolated. Previous reports indicate that human HSC are enriched in Lin-CD34+CD38- cord blood and bone marrow and express CD90. We demonstrate that the Lin-CD34+CD38- fraction of cord blood and bone marrow can be subdivided into three subpopulations: CD90+CD45RA-, CD90-CD45RA-, and CD90-CD45RA+. Utilizing in vivo transplantation studies and complementary in vitro assays, we demonstrate that the Lin-CD34+CD38-CD90+CD45RA- cord blood fraction contains HSC and isolate this activity to as few as 10 purified cells. Furthermore, we report the first prospective isolation of a population of candidate human multipotent progenitors, Lin-CD34+CD38-CD90-CD45RA- cord blood.

PMID:
18371405
PMCID:
PMC2292126
DOI:
10.1016/j.stem.2007.10.001
[Indexed for MEDLINE]
Free PMC Article
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