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Methods Mol Biol. 2008;451:217-27. doi: 10.1007/978-1-59745-102-4_15.

Detection of siRNAs and miRNAs.

Author information

1
Wilhelm Johannsen Center for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, DK-2200 Copenhagen N, Denmark.

Abstract

Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.

PMID:
18370258
DOI:
10.1007/978-1-59745-102-4_15
[Indexed for MEDLINE]

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