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Methods Mol Biol. 2008;432:217-28. doi: 10.1007/978-1-59745-028-7_15.

Use of gas-phase fractionation to increase protein identifications : application to the peroxisome.

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1
ZymoGenetics Inc., Seattle, WA, USA.

Abstract

Gas-phase fractionation (GPF), defined as iterative mass spectrometric interrogations of a sample over multiple smaller mass-to-charge (m/z) ranges, enables the ions selected for collision-induced dissociation to come from a greater number of unique peptides compared to the ions selected from the wide mass range scan in automated liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. GPF is described as a means to achieve higher proteome coverage than multiple LC-MS/MS analyses of unfractionated complex peptide mixtures. It is applied to organellar proteomics through analysis of yeast peroxisomal proteins obtained from a discontinuous Nycodenz gradient fraction known to be enriched with yeast peroxisomal membrane proteins.

PMID:
18370021
DOI:
10.1007/978-1-59745-028-7_15
[Indexed for MEDLINE]
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