Format

Send to

Choose Destination
Anal Biochem. 2008 May 15;376(2):275-6. doi: 10.1016/j.ab.2008.02.022. Epub 2008 Feb 29.

Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue.

Author information

1
Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.

Abstract

A novel procedure for the quantitative isolation and purification of acyl-coenzyme A esters is presented. The procedure involves two steps: (1) tissue extraction using acetonitrile/2-propanol (3+1, v+v) followed by 0.1M potassium phosphate, pH 6.7, and (2) purification using 2-(2-pyridyl)ethyl-functionalized silica gel. Recoveries determined by adding radiolabeled acetyl-, malonyl-, octanoyl-, oleoyl-, palmitoyl-, or arachidonyl-coenzyme A to powdered rat liver varied 93-104% for tissue extraction and 83-90% for solid-phase extraction. The procedure described allows for isolation and purification, with high recoveries, of acyl-coenzyme A esters differing widely in chain length and saturation.

PMID:
18355435
PMCID:
PMC2444051
DOI:
10.1016/j.ab.2008.02.022
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center