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J Clin Virol. 2008 Feb;41(2):134-7.

Epidemiologic and diagnostic evaluation of a recent mumps outbreak using oral fluid samples.

Author information

1
National Virus Reference Laboratory, School of Medicine and Medical Sciences, University College Dublin, Belfield, Dublin 4, Ireland.

Abstract

OBJECTIVE:

To determine the optimal strategy to investigate mumps virus infection in a partially vaccinated cohort.

STUDY DESIGN:

122 oral fluid and serum samples were collected in a recent outbreak in Ireland. The largest age cohort, students aged 18-21 years old attending third level institutions, were investigated using virus isolation, detection of mumps specific IgM, IgG, RT-PCR and molecular genotyping.

RESULTS:

97% of patients had both detectable serum IgM and IgG. Mumps virus RNA was detected in 17 oral fluid samples and 14 of these originated from a single geographic location. Only 6 of the IgM positive samples had detectable mumps virus RNA whereas this could be detected in 11 IgM negative samples. Genotyping studies revealed that genotypes G and J were co-circulating during this outbreak.

CONCLUSIONS:

The use of an oral fluid sample to detect mumps virus RNA and IgM offers a major improvement over serological diagnosis in acute infection in both non-vaccinated or partially vaccinated individuals, and has the advantage that specimens are collected non-invasively.

PMID:
18354822
[Indexed for MEDLINE]

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