The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes

Eduard Nedea; Demet Nalbant; Daniel Xia; Nathaniel T Theoharis; Bernhard Suter; Charles J Richardson; Kelly Tatchell; Thomas Kislinger; Jack F Greenblatt; Peter L Nagy

doi:10.1016/j.molcel.2007.12.031

The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes

Mol Cell. 2008 Mar 14;29(5):577-87. doi: 10.1016/j.molcel.2007.12.031.

Authors

Eduard Nedea¹, Demet Nalbant, Daniel Xia, Nathaniel T Theoharis, Bernhard Suter, Charles J Richardson, Kelly Tatchell, Thomas Kislinger, Jack F Greenblatt, Peter L Nagy

Affiliation

¹ Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada.

PMID: 18342605
DOI: 10.1016/j.molcel.2007.12.031

Abstract

Glc7, the yeast protein phosphatase 1, is a component of the cleavage and polyadenylation factor (CPF). Here we show that downregulation of Glc7, or its dissociation from CPF in the absence of CPF subunits Ref2 or Swd2, results in similar snoRNA termination defects. Overexpressing a C-terminal fragment of Sen1, a superfamily I helicase required for snoRNA termination, suppresses the growth and termination defects associated with loss of Swd2 or Ref2, but not Glc7. Suppression by Sen1 requires nuclear localization and direct interaction with Glc7, which can dephosphorylate Sen1 in vitro. The suppressing fragment, and in a similar manner full-length Sen1, copurifies with the snoRNA termination factors Nrd1 and Nab3, suggesting loss of Glc7 from CPF can be compensated by recruiting Glc7 to Nrd1-Nab3 through Sen1. Swd2 is also a subunit of the Set1c histone H3K4 methyltransferase complex and is required for its stability and optimal methyltransferase activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Death / physiology
DNA Helicases
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Fungal Proteins / genetics
Fungal Proteins / metabolism
Histone-Lysine N-Methyltransferase / genetics
Histone-Lysine N-Methyltransferase / metabolism
Multiprotein Complexes / chemistry
Multiprotein Complexes / metabolism
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism*
Protein Phosphatase 1
Protein Subunits / genetics
Protein Subunits / metabolism*
RNA Helicases
RNA Splicing
RNA, Small Nucleolar / genetics*
RNA, Small Nucleolar / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
Ribonucleoproteins / genetics
Ribonucleoproteins / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription, Genetic*
mRNA Cleavage and Polyadenylation Factors / chemistry
mRNA Cleavage and Polyadenylation Factors / genetics
mRNA Cleavage and Polyadenylation Factors / metabolism*

Substances

DNA-Binding Proteins
Fungal Proteins
Multiprotein Complexes
NAB3 protein, S cerevisiae
NRD1 protein, S cerevisiae
Nuclear Proteins
Protein Subunits
REF2 protein, S cerevisiae
RNA, Small Nucleolar
RNA-Binding Proteins
Ribonucleoproteins
Saccharomyces cerevisiae Proteins
Transcription Factors
mRNA Cleavage and Polyadenylation Factors
Histone-Lysine N-Methyltransferase
SET1 protein, S cerevisiae
SWD2 protein, S cerevisiae
GLC7 protein, S cerevisiae
Phosphoprotein Phosphatases
Protein Phosphatase 1
SEN1 protein, S cerevisiae
DNA Helicases
RNA Helicases