Format

Send to

Choose Destination
Vaccine. 2008 Apr 7;26(16):1942-54. doi: 10.1016/j.vaccine.2008.02.022. Epub 2008 Feb 27.

Identification and characterization of novel antigenic vaccine candidates of Actinobacillus pleuropneumoniae.

Author information

1
Molecular Bacteriology and Immunology Group, Institute of Infection, Immunity & Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom.

Abstract

Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine components. Open reading frames within immuno-reactive phage were analyzed in silico to identify conserved outer membrane proteins. Four ORFs, named comL, lolB, lppC and ompA were chosen for further study. The four encoded proteins were shown experimentally to be antigenic, highly conserved, outer membrane, in vivo-expressed proteins. In pig protection studies, none of the proteins was individually capable of protecting pigs from colonization and infection with the homologous A. pleuropneumoniae strain, despite a detectable specific antibody response being induced.

PMID:
18342410
DOI:
10.1016/j.vaccine.2008.02.022
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center