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Org Lett. 2008 Apr 17;10(8):1561-4. doi: 10.1021/ol702920e. Epub 2008 Mar 14.

Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules.

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1
Department of Chemistry and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA.

Abstract

A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.

PMID:
18338898
DOI:
10.1021/ol702920e
[Indexed for MEDLINE]
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