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Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4283-8. doi: 10.1073/pnas.0712345105. Epub 2008 Mar 12.

Comparative lesion sequencing provides insights into tumor evolution.

Author information

1
The Ludwig Center for Cancer Genetics and Therapeutics, Department of Biostatistics, Howard Hughes Medical Institute, and Sidney Kimmel Cancer Center at The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA.

Abstract

We show that the times separating the birth of benign, invasive, and metastatic tumor cells can be determined by analysis of the mutations they have in common. When combined with prior clinical observations, these analyses suggest the following general conclusions about colorectal tumorigenesis: (i) It takes approximately 17 years for a large benign tumor to evolve into an advanced cancer but <2 years for cells within that cancer to acquire the ability to metastasize; (ii) it requires few, if any, selective events to transform a highly invasive cancer cell into one with the capacity to metastasize; (iii) the process of cell culture ex vivo does not introduce new clonal mutations into colorectal tumor cell populations; and (iv) the rates at which point mutations develop in advanced cancers are similar to those of normal cells. These results have important implications for understanding human tumor pathogenesis, particularly those associated with metastasis.

PMID:
18337506
PMCID:
PMC2393770
DOI:
10.1073/pnas.0712345105
[Indexed for MEDLINE]
Free PMC Article

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