Format

Send to

Choose Destination
Mol Gen Genet. 1991 Oct;229(2):197-205.

Identification of Escherichia coli genes whose expression increases as a function of external pH.

Author information

1
Laboratoire de Microbiologie et de Génétique Moléculaire (UMR CNRS 106), Université Claude Bernard Lyon I, Villeurbanne, France.

Abstract

Using transposon TnphoA and a plate screening method, we have isolated a set of Escherichia coli strains carrying phoA fusions with genes whose expression is modulated as a function of external pH. Besides fusions with the ompF gene and the malB locus, thirteen independent fusions were analysed whose expression is maximal during growth at pHs ranging from 7.0 to 8.5 and minimal during growth at pH 5.0. Six different genetic loci, called phmA, phmB, phmC, phmD, phmE and phmF (for pH modulated) were characterized and localized on the E. coli chromosome at approx. 12, 18, 41, 45, 75 and 84 min, respectively. Expression of phmA::phoA fusions is also influenced when internal pH or environmental conditions such as osmolarity or anaerobiosis are modified. EnvZ protein is not involved in the regulation of phm::phoA fusions.

PMID:
1833617
DOI:
10.1007/bf00272156
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center