Format

Send to

Choose Destination
J Antimicrob Chemother. 2008 May;61(5):1024-8. doi: 10.1093/jac/dkn084. Epub 2008 Mar 11.

The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup.

Author information

1
INSERM U722 and Université Denis Diderot-Paris 7, Faculté de Médecine, Site Xavier Bichat, Paris, France.

Abstract

OBJECTIVES:

A clone of CTX-M-15-producing Escherichia coli has recently been reported to be spreading through Europe and Africa. The aim of this work was to thoroughly characterize this clone.

MATERIALS AND METHODS:

Representative isolates of this clone were subjected to multilocus sequence typing, O typing, virulence gene detection, adhesion assay on human cells, biofilm production assay and mouse lethality assay.

RESULTS:

The clone: (i) belongs to a unique B2 phylogenetic subgroup encompassing the pyelonephritogenic diffusely adhering EC7372 strain; (ii) exhibits a specific O25b molecular subtype; (iii) is identical to the E. coli clone O25:H4-ST131 producing CTX-M-15; (iv) produces biofilm; and (v) is highly virulent in mice despite lacking classical extraintestinal pathogenicity islands (except for high pathogenicity island) and the afa/dra gene.

CONCLUSIONS:

The CTX-M-15-producing E. coli diffusing clone is associated with a high level of antibiotic resistance and with high virulence, showing that, under certain selective pressures, the previously observed trade-off between resistance and virulence may not apply.

PMID:
18334490
DOI:
10.1093/jac/dkn084
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center